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UHPLC and HPLC Compared

Talking of HPLC, High Performance Liquid Chromatography technique had some general benefits that all its users looked forward to with them and these were such as the fact of their high resolution, greatest sensitivity achieved with them and the analysis speeds that they resulted in. Interestingly, these actually became a reality with the coming in of the UHPLC, Ultra High Performance Liquid Chromatography.

This is even looking at the fact that this column technology had proved the fact that column efficiency increased with a reduction in the particle sizes of stationary phases. Looking at the conventional analytical separations, the particle sizes that were used would often be within the ranges of 3 and 5 nanometer sizes. 2 nanometer was seen as a subpar barrier. But this was broken in years that followed and columns packed with the 2 nanometer particle size were shown. This is actually the development that saw the coming in of the UHPLC, Ultra High Performance Chromatography, in column separation in analytical separations.

To understand this better, we will take a look at some of the key differences there are between UHPLC and HPLC.

We will first make a mention of the column dimensions. The UHPLC columns have actually been found to be much shorter and a lot narrower as compared to what would be with the HPLC columns in a general sense. In most cases, you would realize that the HPLC or analytical columns would have dimensions as high as 4.6mm ID by 250mm length while the UHPLC columns can measure even as low as 2.1mm ID by 50mm in length.

Another notable key difference there is between these two is looking at their operating pressure capacities. When we look at the operating pressure factor, we see the fact that the UHPLC columns as well still outdo the HPLC columns, as well known as the analytical columns, where in we see the fact that the UHPLC columns can handle pressures of between 15000 and 18000 psi while the HPLC columns can only handle such pressures within a system between 5000 psi and 6000 psi as their maximum limits. Actually, the pumps will be able to handle such extreme pressure conditions with the least of effects of wear and tear on their parts.

Not to forget mention the fact of better detection with the UHPLC columns. This can be explained by the fact that the UHPLC columns produce narrow peaks, which are often a few seconds or less, as compared to the conventional HPLC or analytical separations or columns that have such a slow response and as such generate some sort of compressed leaks as a result of lesser number of their data points making them somehow inaccurate in their detection in the end.

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